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Sample GSM1373728 Query DataSets for GSM1373728
Status Public on Jan 01, 2016
Title Wild-type Male 1
Sample type RNA
 
Source name Wild-type_male_lung
Organism Mus musculus
Characteristics strain background: C57/BL6
genotype/variation: Wild-type
gender: male
age: ~28-32 weeks
tissue: lung
Treatment protocol none
Growth protocol Mice were housed in the same animal facility with access to same food and water.
Extracted molecule total RNA
Extraction protocol RNA was isolated according to Qiagen RNAeasy Kit per manufacturers protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000 7G
Description WM1
Data processing The data were analyzed using the Bioconductor package in R to perform RMA normalization.
 
Submission date Apr 24, 2014
Last update date Jan 01, 2016
Contact name Augustine MK Choi
Organization name Weill Cornell Medical College
Department Department of Medicine
Street address 525 East 68th Street, Room M-522, Box 130
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL17777
Series (1)
GSE57048 Irp2 mediates cigarette smoke-induced bronchitis and emphysema via regulation of cytochrome c oxidase and mitochondrial iron loading.

Data table header descriptions
ID_REF
VALUE RMA-normalized expression value.

Data table
ID_REF VALUE
100008567_at 9.966806653
100009600_at 5.995537932
100009609_at 3.963072827
100009614_at 7.14314364
100012_at 5.06207224
100017_at 9.845687068
100019_at 8.778336062
100033459_at 7.018574232
100034251_at 10.23930805
100034748_at 6.925621449
100036520_at 7.285452432
100036521_at 7.948892372
100036523_at 7.257273638
100036537_at 6.411061676
100036768_at 6.27416812
100037258_at 10.98353752
100037260_at 7.27209057
100037262_at 7.912782429
100037278_at 7.8270492
100037396_at 6.593302034

Total number of rows: 21187

Table truncated, full table size 442 Kbytes.




Supplementary file Size Download File type/resource
GSM1373728_AC2012101238.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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