|
| Status |
Public on Feb 20, 2015 |
| Title |
Microglia_Ab_WT_4 |
| Sample type |
RNA |
| |
|
| Source name |
Primary microglia from 8 month-old mice
|
| Organism |
Mus musculus |
| Characteristics |
treatment: I.C.V. Aβ42 fibrillar 48h prior to cell isolation
genotype: CD11b-Cre ; EP2+/+
strain: C57BL/6
age: 8 month-old
|
| Treatment protocol |
Primary microglia were from mice that 48h before had received I.C.V. injections of Aβ or vehicle.
|
| Growth protocol |
Primary microglia were isolated directly from adult mouse brain and processed immediately for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol followed by Qiagen RNeasy kit extraction according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Performed by the Stanford University PAN facility using the GeneChip Whole Transcript Sense Target Labeling Assay from Affymetrix (P/N 701880)
|
| |
|
| Hybridization protocol |
Performed by the Stanford University PAN facility using the GeneChip Hybridization protocol from Affymetrix (P/N 702731)
|
| Scan protocol |
Performed by the Stanford University PAN facility using a GCS 3000 Scanner and Gene Chip Command Console software
|
| Data processing |
The data were analyzed using Partek Genomics Suite version 6.6, using RMA default settings.
|
| |
|
| Submission date |
Apr 30, 2014 |
| Last update date |
Feb 20, 2015 |
| Contact name |
Katrin Andreasson |
| E-mail(s) |
kandreas@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Neurology and Neurological Sciences
|
| Street address |
1201 Welch Road, MSLS P210
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE57181 |
In vivo response of microglia to Aβ and prostaglandin-E2 EP2 receptor signaling |
|
