|
| Status |
Public on May 07, 2014 |
| Title |
male pupae replicate 2 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
male Drosophila pupae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Sex: male
developmental stage: pupae
|
| Treatment protocol |
Flies laid in staggered vials and larvae, pupae and adults samples collected at the same time from vials at the right stage of development. 10 individuals included in each biological replicate collected.
|
| Growth protocol |
LHm outbred population kept at 25oC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ambion miRvana total RNA extraction kit used according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Affymetrix One-Cycle Target Labeling (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix One-Cycle Target Labeling (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Scan protocol |
Affymetrix One-Cycle Target Labeling (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Description |
gene expression data from LHm outbred population
|
| Data processing |
RMA standard
|
| |
|
| Submission date |
May 06, 2014 |
| Last update date |
May 07, 2014 |
| Contact name |
Fiona C Ingleby |
| Organization name |
University of Sussex
|
| Department |
School of Biology
|
| Street address |
John Maynard Smith Building
|
| City |
Falmer |
| ZIP/Postal code |
BN1 9QG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16384 |
| Series (2) |
| GSE57321 |
Sex-specific mRNA and miRNA expression data from Drosophila larvae, pupae and adults [miRNA] |
| GSE57322 |
Sex-specific mRNA and miRNA expression data from Drosophila larvae, pupae and adults |
|
