|
| Status |
Public on Jan 01, 2015 |
| Title |
Infected_24h pi_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CHME3 cells grown on transwells, infected with JEV, 24h pi
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CHME3
cell type: microglial cell line
cell state: polarized
treatment: Japanese encephalitis virus strain P20778 infection
time point: 24h post infection
|
| Treatment protocol |
Infected with an MOI of 5 pfu/cell of Japanese encephalitis virus (P20778 strain).
|
| Growth protocol |
CHME3 cells grown on 6-well plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared using Qiagen RNA extraction kit.
|
| Label |
biotin
|
| Label protocol |
Complex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Complex probes were hybridized to each GeneChip® miRNA 3.0 array according to the manufacturer's recommendations (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
SAMPLE 4A
miRNA microarray data.
|
| Data processing |
Scanned images of microarray chips were analyzed using Affymetrix Expression Console with the default RMA settings. Raw data were processed and normalized on basis of organism human using RMA algorithm. The normalized data (CHP files) processed for the differential miRNA analysis in Affymetrix TAC software.
|
| |
|
| Submission date |
May 07, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
ARUP BANERJEE |
| E-mail(s) |
banerjeea@thsti.res.in
|
| Phone |
9650328080
|
| Organization name |
TRANSLATIONAL HEALTH SCIENCE & TECHNOLOGY INSTITUTE
|
| Department |
VIDRC
|
| Lab |
THSTI, VIDRC
|
| Street address |
THSTI, 3RD MILE STONE, FARIDABAD GURGAON EXPRESSWAY, NCR BIOTECH CLUSTER
|
| City |
FARIDABAD |
| State/province |
Haryana |
| ZIP/Postal code |
121001 |
| Country |
India |
| |
|
| Platform ID |
GPL16384 |
| Series (2) |
| GSE57410 |
MicroRNA expression profile in CHME3 cells infected with Japanese encephalitis virus |
| GSE57647 |
Time- and phase-dependent expression profile of miRNA and mRNA in human microglial cells (CHME3) infected with Japanese encephalitis virus (JEV) P20778 strain |
|
