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Sample GSM1382249 Query DataSets for GSM1382249
Status Public on Jan 01, 2015
Title Infected_48h pi_Rep3
Sample type RNA
 
Source name CHME3 cells grown on transwells, infected with JEV, 48h pi
Organism Homo sapiens
Characteristics cell line: CHME3
cell type: microglial cell line
cell state: polarized
treatment: Japanese encephalitis virus strain P20778 infection
time point: 48h post infection
Treatment protocol Infected with an MOI of 5 pfu/cell of Japanese encephalitis virus (P20778 strain).
Growth protocol CHME3 cells grown on 6-well plates.
Extracted molecule total RNA
Extraction protocol Total RNA prepared using Qiagen RNA extraction kit.
Label biotin
Label protocol Complex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA).
 
Hybridization protocol Complex probes were hybridized to each GeneChip® miRNA 3.0 array according to the manufacturer's recommendations (Affymetrix).
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description SAMPLE 6C
miRNA microarray data.
Data processing Scanned images of microarray chips were analyzed using Affymetrix Expression Console with the default RMA settings. Raw data were processed and normalized on basis of organism human using RMA algorithm. The normalized data (CHP files) processed for the differential miRNA analysis in Affymetrix TAC software.
 
Submission date May 07, 2014
Last update date Jan 01, 2015
Contact name ARUP BANERJEE
E-mail(s) banerjeea@thsti.res.in
Phone 9650328080
Organization name TRANSLATIONAL HEALTH SCIENCE & TECHNOLOGY INSTITUTE
Department VIDRC
Lab THSTI, VIDRC
Street address THSTI, 3RD MILE STONE, FARIDABAD GURGAON EXPRESSWAY, NCR BIOTECH CLUSTER
City FARIDABAD
State/province Haryana
ZIP/Postal code 121001
Country India
 
Platform ID GPL16384
Series (2)
GSE57410 MicroRNA expression profile in CHME3 cells infected with Japanese encephalitis virus
GSE57647 Time- and phase-dependent expression profile of miRNA and mRNA in human microglial cells (CHME3) infected with Japanese encephalitis virus (JEV) P20778 strain

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
14q0_st 1.065282
14qI-1_st 2.136514
14qI-1_x_st 2.888231
14qI-2_st 1.143199
14qI-3_x_st 1.217607
14qI-4_st 1.260226
14qI-4_x_st 1.429371
14qI-5_st 0.9923701
14qI-6_st 1.211912
14qI-6_x_st 1.320383
14qI-7_st 1.464951
14qI-8_st 1.145793
14qI-8_x_st 1.159966
14qI-9_x_st 1.000195
14qII-10_st 1.035456
14qII-10_x_st 1.008276
14qII-11_st 2.280979
14qII-11_x_st 1.42096
14qII-12_st 1.127088
14qII-12_x_st 0.9539139

Total number of rows: 25015

Table truncated, full table size 638 Kbytes.




Supplementary file Size Download File type/resource
GSM1382249_6C.CEL.gz 948.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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