|
| Status |
Public on Jan 01, 2015 |
| Title |
Dose_1/5 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HOS_MEM media treated with sodium fluoride
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HOS
cell type: Osetoblast cells
treated with: 1/5th conc. of 24h LC50 of sodium fluoride
time point: after 1 month of chronic exposure
|
| Treatment protocol |
Treated with 1/5th and 1/2 concentration of 24h LC50 of sodium fluoride.
|
| Growth protocol |
HOS cells grown on MEM media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared using STRATAGENE ABSOLUTELY RNA KIT
|
| Label |
biotin
|
| Label protocol |
Complex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA)Affymetrix)
|
| |
|
| Hybridization protocol |
Complex probes were hybridized to each GeneChip® miRNA 3.0 array according to the manufacturer's recommendations (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Dose_1_5 2
|
| Data processing |
The data were analyzed with Expression Console and Gene springGX 12.0. For the data normalization, Affymetrix default analysis settings and RMA+DABG All organism was selected. The probe set reprsents fonly or the human from the normalized data had been included and imported in GeneSpring for differnetiall miRNA analysis followed by intensities less than the 20.0 percentile in the normalized data were excluded.
|
| |
|
| Submission date |
May 12, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
praveen Kumar Naoghare |
| E-mail(s) |
pinbiotech@gmail.com
|
| Phone |
911244779800
|
| Organization name |
Innovative Life Discoveries
|
| Department |
Environmental Health Division
|
| Lab |
CSIR Lab
|
| Street address |
CSIR Lab,Nehru Marg
|
| City |
Nagpur |
| State/province |
Maharashtra |
| ZIP/Postal code |
440020 |
| Country |
India |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE57550 |
miRNA expression profile in HOS cells exposed to sodium fluoride |
|
