|
| Status |
Public on Jul 30, 2014 |
| Title |
BMM_RANKL 1day_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow-derived macrophage cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell culture type: Primary cells
cell type: Bone marrow-derived macrophage cells
age: 6-8 weeks
|
| Treatment protocol |
To generate osteoclasts, BMMs were cultured in the presence of M-CSF (30 ng/ml) and RANKL (50 ng/ml).
|
| Growth protocol |
Bone marrow (BM) cells were collected by flushing femurs and tibias from 6- to 8-week-old C57BL/6 mice. Cells were cultured in α-minimum essential medium (α-MEM) supplemented with 10% FBS and M-CSF (5 ng/ml) for 16 h. Non-adherent cells were harvested and cultured with M-CSF (30 ng/ml) for 3 days. Floating cells were removed and adherent cells were used as bone marrow-derived macrophage (BMM) cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the TRIzol reagent according to the manufacturer's instructinos.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina array.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 2
|
| Data processing |
The data were processed and anlayzed by the Array Pipe software.
|
| |
|
| Submission date |
May 15, 2014 |
| Last update date |
Jul 30, 2014 |
| Contact name |
Kyunghwan Kim |
| E-mail(s) |
kyunghki@usc.edu
|
| Phone |
3234424477
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
1450 Biggy Street NRT6517
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE57468 |
Genome-wide expression profiling in bone marrow-derived macrophage (BMM) cells upon RANKL treatment |
|
