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Sample GSM1387476 Query DataSets for GSM1387476
Status Public on May 21, 2015
Title Meg-3_2
Sample type RNA
 
Source name MIN6-4N_Meg3_overexpression
Organism Mus musculus
Characteristics cell line: MIN6-4N
cell type: pancreatic beta cell
Treatment protocol MIN6-4N cells were transfected with Vector or Meg3 plasmid using the nucleofection method with Solution -T (Amaxa/Lonza) and selected for 5 weeks in 750 µg/ml G418. Stable clones were picked by using cloning cylinders and expanded, and tested for Meg3 expression by RT-PCR.
Growth protocol MIN6-4N stable cell lines cells were cultured in DMEM low glucose medium supplemented with 15% FBS, 1X antibiotic and antimycotic, and 500 µg/ml G418 at 37˚C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAeasy kit according to the manufacturer's instructions (Qiagen) from 3 independent cultures of a vector transfected clone (Vec-3), and a Meg3 transfected clone (Meg-5).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description gene expression upon stable mouse Meg3 expression
The sorted cell subpopulations
Data processing RMA by software Partek
 
Submission date May 15, 2014
Last update date May 21, 2015
Contact name WeiPing Chen
E-mail(s) weipingChen@niddk.nih.gov
Phone 301-496-0175
Organization name NIDDK/NIH
Department GCL
Lab Genomics Core Lab
Street address Bldg 8, Room 1A11, NIDDK/NIH
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10740
Series (1)
GSE57716 Meg3 regulated genes in pancreatic beta cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
10338068 5.67441
10338069 5.06818
10338070 3.73716
10338071 4.04046
10338072 5.24831
10338073 4.899
10338074 9.59966
10338075 8.54732
10338076 9.08025
10338077 9.3592
10338078 7.87344
10338079 4.01263
10338080 4.47087
10338081 5.98293
10338082 7.19921
10338083 4.00296
10338084 6.02326
10338085 9.12222
10338086 6.21537
10338087 5.83792

Total number of rows: 241419

Table truncated, full table size 3982 Kbytes.




Supplementary file Size Download File type/resource
GSM1387476_11-26-13_SAgarwal5_Mo_Gene_1_0_ST_M5-2.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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