|
| Status |
Public on Aug 20, 2014 |
| Title |
sorted spleenic WT CD4+ T cells rep2 |
| Sample type |
RNA |
| |
|
| Source name |
WT CD4+ (CD45.1) T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6 mixed bone marrow chimera
age: 12 months
tissue: spleen
cell type: WT CD4+ T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
WT sample2
|
| Data processing |
The data were normalised using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
May 16, 2014 |
| Last update date |
Aug 20, 2014 |
| Contact name |
Bernardo Reis |
| E-mail(s) |
breis@rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Street address |
1234 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE57738 |
T cell-intrinsic MyD88 signaling in cognate immune response to intracellular parasite infection: crucial role for IL-18R |
|
