|
| Status |
Public on Sep 01, 2014 |
| Title |
NTKO3 |
| Sample type |
RNA |
| |
|
| Source name |
NTKO cells
|
| Organism |
Homo sapiens |
| Characteristics |
parental cell line: KMS11
cell type: multiple myeloma cell line
genotype/variation: wild type MMSET allele inactivated
|
| Growth protocol |
Cells were grown in RPMI 1640 supplemented with 10% FBS and 1% Pen/Strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit.
|
| Label |
Cy3
|
| Label protocol |
Biotin-UTP labeled using the Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to the Illumina HumanWG-6 v3.0 Expression BeadChip using standard protocols (provided by Illumina).
|
| Scan protocol |
The arrays were scanned on an Illumina BeadArray Reader.
|
| Description |
Replicate 3 of 3.
|
| Data processing |
The data was processed using GenomeStudio.
|
| |
|
| Submission date |
May 21, 2014 |
| Last update date |
Sep 01, 2014 |
| Contact name |
Relja Popovic |
| E-mail(s) |
r-popovic@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
303 East Superior Street
|
| City |
Chicago |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE57863 |
MMSET regulation of gene expression in t(4;14)+ myeloma |
|
