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Sample GSM1396552 Query DataSets for GSM1396552
Status Public on May 21, 2017
Title PC2FiOPCp25 rep 2
Sample type RNA
 
Source name PC2FiOPCp25 rep 2
Organism Mus musculus
Characteristics cell type: iOPC
strain: C57BL/6
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 18h)
The RNA samples from in vivo transplanted and FACS sorted cells
were amplified using the TargetAmp 2-Round Biotin-aRNA Amplification Kit 3.0 (Epicentre
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Purified and labeled cRNA was then hybridized for 18 hrs onto MouseRef-8 v2 expression BeadChips (Illumina
Scan protocol After the recommended washing
the chips were stained with streptavidin-Cy3 (GE Healthcare) and scanned using the iScan reader (Illumina) and the accompanying software.
Description replicate 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina)
background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model
variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Data post-processing and graphics were performed with in-house developed functions in MATLAB.
 
Submission date May 22, 2014
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE57890 Pericyte Characteristics and an Optimal Factor Combination Enable Generating Bona-Fide iOPCs with the High Efficiency

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 1.835192
ILMN_1212612 1.234124
ILMN_1212619 4.893004
ILMN_1212628 1.305426
ILMN_1212632 5.505447
ILMN_1212636 10.621047
ILMN_1212637 9.501429
ILMN_1212645 1.545154
ILMN_1212648 8.095471
ILMN_1212653 10.413172
ILMN_1212672 7.624306
ILMN_1212682 2.343445
ILMN_1212683 1.382581
ILMN_1212685 2.043694
ILMN_1212692 3.769326
ILMN_1212693 6.602403
ILMN_1212695 2.073144
ILMN_1212698 1.428523
ILMN_1212702 1.247738
ILMN_1212703 6.059985

Total number of rows: 25697

Table truncated, full table size 553 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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