|
| Status |
Public on Nov 11, 2014 |
| Title |
OC2-WT adult 1 |
| Sample type |
RNA |
| |
|
| Source name |
OC2-WT adult retina
|
| Organism |
Mus musculus |
| Characteristics |
tissue: retina
Stage: adult
onecut genotype: WT
|
| Treatment protocol |
Retinas were isolated from adult eyes and rinsed in 1X PBS.
|
| Growth protocol |
Littermates were allowed to develop normally in utero until maturity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trireagent extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA wa amplified, biotinylated, and fragmented using the Ambion MessageAmp III RNA amplification kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Amplified RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
|
| Scan protocol |
Amplified RNA was scanned from Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
|
| Description |
Gene expression information from isolated adult retinas
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 22, 2014 |
| Last update date |
Nov 11, 2014 |
| Contact name |
Jeffrey M Trimarchi |
| E-mail(s) |
jtrimarc@iastate.edu
|
| Phone |
2172190210
|
| Organization name |
Iowa State University
|
| Department |
GDCB
|
| Lab |
Trimarchi
|
| Street address |
3188 Molecular Biology Building
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50014 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE57918 |
Expression data from adult Onecut2 WT and KO animals |
|
