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Sample GSM1397411 Query DataSets for GSM1397411
Status Public on Nov 11, 2014
Title OC2-WT adult 1
Sample type RNA
 
Source name OC2-WT adult retina
Organism Mus musculus
Characteristics tissue: retina
Stage: adult
onecut genotype: WT
Treatment protocol Retinas were isolated from adult eyes and rinsed in 1X PBS.
Growth protocol Littermates were allowed to develop normally in utero until maturity
Extracted molecule total RNA
Extraction protocol Trireagent extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA wa amplified, biotinylated, and fragmented using the Ambion MessageAmp III RNA amplification kit according to manufacturer's instructions.
 
Hybridization protocol Amplified RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
Scan protocol Amplified RNA was scanned from Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
Description Gene expression information from isolated adult retinas
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date May 22, 2014
Last update date Nov 11, 2014
Contact name Jeffrey M Trimarchi
E-mail(s) jtrimarc@iastate.edu
Phone 2172190210
Organization name Iowa State University
Department GDCB
Lab Trimarchi
Street address 3188 Molecular Biology Building
City Ames
State/province IA
ZIP/Postal code 50014
Country USA
 
Platform ID GPL1261
Series (1)
GSE57918 Expression data from adult Onecut2 WT and KO animals

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 868.228 P 5.16732e-05
AFFX-BioB-M_at 1581.72 P 4.42873e-05
AFFX-BioB-3_at 986.1 P 4.42873e-05
AFFX-BioC-5_at 2423.18 P 6.02111e-05
AFFX-BioC-3_at 3020.57 P 5.16732e-05
AFFX-BioDn-5_at 5514.96 P 5.16732e-05
AFFX-BioDn-3_at 9389.73 P 7.00668e-05
AFFX-CreX-5_at 28514.6 P 5.16732e-05
AFFX-CreX-3_at 29590.3 P 4.42873e-05
AFFX-DapX-5_at 3.15918 A 0.737173
AFFX-DapX-M_at 12.8094 A 0.699394
AFFX-DapX-3_at 2.46573 A 0.969024
AFFX-LysX-5_at 0.606859 A 0.995005
AFFX-LysX-M_at 9.0143 A 0.737173
AFFX-LysX-3_at 20.9401 A 0.0956669
AFFX-PheX-5_at 3.77816 A 0.932322
AFFX-PheX-M_at 5.37577 A 0.81489
AFFX-PheX-3_at 5.78137 A 0.804734
AFFX-ThrX-5_at 6.61663 A 0.603098
AFFX-ThrX-M_at 4.24097 A 0.772364

Total number of rows: 45101

Table truncated, full table size 1377 Kbytes.




Supplementary file Size Download File type/resource
GSM1397411_WT_adult_OC2_1.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM1397411_WT_adult_OC2_1.mas5.CHP.gz 399.6 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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