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Sample GSM1397412 Query DataSets for GSM1397412
Status Public on Nov 11, 2014
Title OC2-WT adult 2
Sample type RNA
Source name OC2-WT adult retina
Organism Mus musculus
Characteristics tissue: retina
Stage: adult
onecut genotype: WT
Treatment protocol Retinas were isolated from adult eyes and rinsed in 1X PBS.
Growth protocol Littermates were allowed to develop normally in utero until maturity
Extracted molecule total RNA
Extraction protocol Trireagent extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA wa amplified, biotinylated, and fragmented using the Ambion MessageAmp III RNA amplification kit according to manufacturer's instructions.
Hybridization protocol Amplified RNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
Scan protocol Amplified RNA was scanned from Affymetrix GeneChip Mouse Genome 430 2.0 arrays at the Iowa State University GeneChip Facility.
Description Gene expression information from isolated adult retinas
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date May 22, 2014
Last update date Nov 11, 2014
Contact name Jeffrey M Trimarchi
Phone 2172190210
Organization name Iowa State University
Department GDCB
Lab Trimarchi
Street address 3188 Molecular Biology Building
City Ames
State/province IA
ZIP/Postal code 50014
Country USA
Platform ID GPL1261
Series (1)
GSE57918 Expression data from adult Onecut2 WT and KO animals

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 507.166 P 0.000169227
AFFX-BioB-M_at 810.784 P 4.42873e-05
AFFX-BioB-3_at 564.733 P 4.42873e-05
AFFX-BioC-5_at 1271.39 P 5.16732e-05
AFFX-BioC-3_at 1731.32 P 4.42873e-05
AFFX-BioDn-5_at 3406.35 P 4.42873e-05
AFFX-BioDn-3_at 6336.74 P 7.00668e-05
AFFX-CreX-5_at 16739.1 P 5.16732e-05
AFFX-CreX-3_at 17087.2 P 4.42873e-05
AFFX-DapX-5_at 9.55013 A 0.712274
AFFX-DapX-M_at 4.92302 A 0.883887
AFFX-DapX-3_at 3.54781 A 0.852082
AFFX-LysX-5_at 0.539143 A 0.99679
AFFX-LysX-M_at 3.6302 A 0.804734
AFFX-LysX-3_at 12.6886 A 0.156732
AFFX-PheX-5_at 17.7481 A 0.440646
AFFX-PheX-M_at 2.44828 A 0.883908
AFFX-PheX-3_at 7.40796 A 0.574038
AFFX-ThrX-5_at 5.95554 A 0.544587
AFFX-ThrX-M_at 17.1465 A 0.340648

Total number of rows: 45101

Table truncated, full table size 1391 Kbytes.

Supplementary file Size Download File type/resource
GSM1397412_WT_adult_OC2_2.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM1397412_WT_adult_OC2_2.mas5.CHP.gz 395.5 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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