|
| Status |
Public on Oct 13, 2007 |
| Title |
129 Mouse P0 Pineal Tissue, Sample 3, 05-31-06 |
| Sample type |
RNA |
| |
|
| Source name |
Wildtype, 129 mouse pineal glands from postnatal day zero.
|
| Organism |
Mus musculus |
| Characteristics |
129 mouse pineal gland tissues taken at postnatal day zero.
|
| Biomaterial provider |
Klein Lab
|
| Treatment protocol |
Pineal tissues collected during the daytime period.
3 pineal glands were pooled per sample and total RNA extracted.
|
| Growth protocol |
Mice housed in LD 12:12 light cycles.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Initially, total RNA was subjected to a single round of RNA amplification using Ambion Message Amp II kit.
Amplified RNA was used as starting material for the Affymetrix hybridization protocol.
|
| Label |
Biotin
|
| Label protocol |
3' In Vitro Transcription, GeneChip® One-Cycle Target Labeling from Affymetrix.
|
| |
|
| Hybridization protocol |
GeneChip® One-Cycle Target Labeling from Affymetrix.
Fluidics script EukGE_Ws2v5 was used with GeneChip® Fluidics Station 450.
|
| Scan protocol |
Affymetrix GCOS default settings and Genechip Scanner 3000.
|
| Description |
This is WT sample number three of three.
Experiment compares 129 Wt pineal gland gene expression at P0 versus P0 pineal gland gene expression in NeuroD1 knockout mice.
|
| Data processing |
Data indicated are the signal calls from GCOS
|
| |
|
| Submission date |
Oct 13, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Michael Bailey |
| E-mail(s) |
baileymi@mail.nih.gov
|
| Phone |
301-451-6627
|
| Fax |
301-480-3526
|
| Organization name |
National Institutes of Health
|
| Department |
NICHD
|
| Lab |
Section on Neuroendocrinology
|
| Street address |
9000 Rockville Pike, Bldg 49, Rm 6A71
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20814 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6030 |
Pineal gland gene expression of 129 Wt P0 mice versus NeuroD1 pineal gene expression at P0 |
|
| Relations |
| Reanalyzed by |
GSE119085 |
