|
Status |
Public on Oct 05, 2007 |
Title |
MEF_CDKO_1OHT_2 |
Sample type |
RNA |
|
|
Source name |
Mouse embryonic Fibroblast Conditional Dicer Knockout 1 uM Tamoxifen 2
|
Organism |
Mus musculus |
Characteristics |
Mouse embryonic Fibroblast derived from a conditional Dicer knockout mouse Embryos were at developmental stage E16
|
Treatment protocol |
MEFs were either non treated or treated with 4-Orthohydroxy Tamoxifen (1 or 5 uM) for 4 days then RNA was extracted using Trizol Reagent
|
Growth protocol |
DMEM 10% FBS +L-Glutamine +D-Glucose in standard cell culture conditions
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
According to the standard Affymetrix protocol
|
Label protocol |
According to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
According to the standard Affymetrix protocol
|
Scan protocol |
According to the standard Affymetrix protocol
|
Description |
MEF with a conditional Dicer knockout gene
|
Data processing |
The raw CEL files were analyzed using gcrma package in bioconductor (R statistical language) using default settings. The VALUES are log2 expression levels.
|
|
|
Submission date |
Oct 16, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Noam Shomron |
E-mail(s) |
nshomron@mit.edu
|
Phone |
6172536726
|
Organization name |
Massachusetts Institute of Technology
|
Department |
Biology
|
Lab |
Christopher Burge
|
Street address |
77 Massachusetts Ave 68-217
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02139 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE6046 |
Expression data from Dicer knockout MEF |
|
Relations |
Reanalyzed by |
GSE119085 |