|
| Status |
Public on Oct 05, 2007 |
| Title |
MEF_CDKO_5OHT_2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic Fibroblast Conditional Dicer Knockout 5 uM Tamoxifen 2
|
| Organism |
Mus musculus |
| Characteristics |
Mouse embryonic Fibroblast derived from a conditional Dicer knockout mouse
Embryos were at developmental stage E16
|
| Treatment protocol |
MEFs were either non treated or treated with 4-Orthohydroxy Tamoxifen (1 or 5 uM) for 4 days then RNA was extracted using Trizol Reagent
|
| Growth protocol |
DMEM 10% FBS +L-Glutamine +D-Glucose in standard cell culture conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
According to the standard Affymetrix protocol
|
| Label protocol |
According to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
According to the standard Affymetrix protocol
|
| Scan protocol |
According to the standard Affymetrix protocol
|
| Description |
MEF with a conditional Dicer knockout gene
|
| Data processing |
The raw CEL files were analyzed using gcrma package in bioconductor (R statistical language) using default settings. The VALUES are log2 expression levels.
|
| |
|
| Submission date |
Oct 16, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Noam Shomron |
| E-mail(s) |
nshomron@mit.edu
|
| Phone |
6172536726
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Christopher Burge
|
| Street address |
77 Massachusetts Ave 68-217
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6046 |
Expression data from Dicer knockout MEF |
|
| Relations |
| Reanalyzed by |
GSE119085 |
