|
| Status |
Public on Jun 10, 2014 |
| Title |
methanol-treated 2 |
| Sample type |
RNA |
| |
|
| Source name |
methanol-treated 2
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
tissue: brain
|
| Treatment protocol |
120 mg/kg methanol (350 mcL) and 350 mcL of normal saline solution
|
| Growth protocol |
-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jun 09, 2014 |
| Last update date |
Jun 10, 2014 |
| Contact name |
Anastasia Shindyapina |
| E-mail(s) |
shindyapina.a@gmail.com
|
| Organization name |
MSU Lomonosov
|
| Department |
Biengineering
|
| Street address |
Mosfilmovskaya 76
|
| City |
Moscow |
| State/province |
Moscow |
| ZIP/Postal code |
119192 |
| Country |
Russia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE58303 |
Mouse brain tissue after methanol and normal saline treatment |
|
