|
Status |
Public on Jun 10, 2014 |
Title |
normal saline 4 |
Sample type |
RNA |
|
|
Source name |
normal saline 4
|
Organism |
Mus musculus |
Characteristics |
gender: male tissue: brain
|
Treatment protocol |
120 mg/kg methanol (350 mcL) and 350 mcL of normal saline solution
|
Growth protocol |
-
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
|
|
Submission date |
Jun 09, 2014 |
Last update date |
Jun 10, 2014 |
Contact name |
Anastasia Shindyapina |
E-mail(s) |
shindyapina.a@gmail.com
|
Organization name |
MSU Lomonosov
|
Department |
Biengineering
|
Street address |
Mosfilmovskaya 76
|
City |
Moscow |
State/province |
Moscow |
ZIP/Postal code |
119192 |
Country |
Russia |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE58303 |
Mouse brain tissue after methanol and normal saline treatment |
|