|
Status |
Public on Dec 08, 2015 |
Title |
R EtOH rep3 hyb1 |
Sample type |
RNA |
|
|
Source name |
ESCs
|
Organism |
Mus musculus |
Characteristics |
treatment: EtOH hyb number: 1
|
Treatment protocol |
Tamoxifen was added at 1uM for 36h to induce knockout
|
Growth protocol |
All cells were grown in Knockout DMEM with 15%FBS, Pen/Strep, L-glutamine and Non-essential amino acids. ESCs were also grown in Lif and 2i (GSKi and MEKi).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with the standard Trizol protocol
|
Label |
biotin
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Standard Illumina protocol - a subset of the RNA was hybed on two separate chips at different times and used as such on Sample Network (see hyb#)
|
Scan protocol |
Standard Illumina protocol
|
Data processing |
Using RGui, the data was processed by Sample Network (ref) and subsequently quantile normalized. Differential gene expression was performed using the limma package, with a cutoff of adjusted p-value < 0.05.
|
|
|
Submission date |
Jun 09, 2014 |
Last update date |
Dec 08, 2015 |
Contact name |
Raga Krishnakumar |
E-mail(s) |
raga.krishnakumar@gmail.com
|
Organization name |
UCSF
|
Lab |
Blelloch
|
Street address |
912E RMB, 35 Medical Center Way
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE58317 |
Foxd3 binding at enhancers controls developmental timing of gene expression by regulation of histone acetylation [expression array] |
GSE58408 |
Foxd3 binding at enhancers controls developmental timing of gene expression by regulation of histone acetylation |
|