|
Status |
Public on Jun 17, 2014 |
Title |
obese-control_replicate2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
muscle obese control
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Zucker tissue: skeletal muscle gender: male
|
Extracted molecule |
total RNA |
Extraction protocol |
For miRNA microarray and qRT-PCR analysis total RNA, including small RNAs, was isolated from muscle samples using the Qiagen miRNeasy Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol.
|
Label |
Hy3
|
Label protocol |
750 ng total RNA from both sample and reference was labeled using the mercury LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark).
|
|
|
Channel 2 |
Source name |
muscle obese control
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Zucker tissue: skeletal muscle gender: male
|
Extracted molecule |
total RNA |
Extraction protocol |
For miRNA microarray and qRT-PCR analysis total RNA, including small RNAs, was isolated from muscle samples using the Qiagen miRNeasy Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol.
|
Label |
Hy5
|
Label protocol |
750 ng total RNA from both sample and reference was labeled using the mercury LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark).
|
|
|
|
Hybridization protocol |
Labeled RNA samples were mixed pair-wise and hybridized according to the instruction manual using a Tecan HS4800TM hybridization station (Tecan, Austria).
|
Scan protocol |
Afterwards, slides were scanned using the Agilent G2565BA Microarray scanner System (Agilent Technologies, Inc., USA).
|
Description |
Biological replicate 2 from 6. obese control sample number in normalized data file: 1
|
Data processing |
The image analysis was carried out using the ImaGene® 9 (mercury LNATM microRNA Array Analysis Software, Exiqon, Denmark). The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm. The file "Normalized_data.txt contains the normalized signal intensities.
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|
|
Submission date |
Jun 16, 2014 |
Last update date |
Jun 17, 2014 |
Contact name |
Janine Keller |
E-mail(s) |
janine.keller1@gmx.net
|
Organization name |
JLU Giessen
|
Department |
Inst. for Animal Nutrition
|
Street address |
Heinrich-Buff-Ring 26-32
|
City |
Giessen |
ZIP/Postal code |
35392 |
Country |
Germany |
|
|
Platform ID |
GPL17107 |
Series (1) |
GSE58537 |
Supplemental carnitine affects the microRNA expression profile in skeletal muscle of obese Zucker rats |
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