|
| Status |
Public on Nov 07, 2006 |
| Title |
mouse brain antisense U74B |
| Sample type |
RNA |
| |
|
| Source name |
total mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
Strain 129SvJ
Male, age 5 weeks
|
| Treatment protocol |
The brain was dissected, rinsed in ice cold PBS and directly used for RNA extraction with TRIzol (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip? Scanner 3000 (Affymetrix).
|
| Description |
Antisense transcripts: Reversely annotated probesets identified using the mask provided by Affymetrix to distinguish beween reverse and forward probe sets.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 23, 2006 |
| Last update date |
Nov 06, 2006 |
| Contact name |
Andreas Werner |
| E-mail(s) |
andreas.werner@ncl.ac.uk
|
| Phone |
+44 191 222 6990
|
| Organization name |
Newcastle University
|
| Department |
Cell and Molecular Biosciences
|
| Street address |
Framlington Place
|
| City |
Newcastle |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL33 |
| Series (1) |
| GSE6106 |
Sense-antisense transcript comparison in mouse brain and kidney |
|
