|
| Status |
Public on Oct 24, 2006 |
| Title |
HF1077_Hind_2 |
| Sample type |
genomic |
| |
|
| Source name |
Brain tissue (snap-frozen sections) from glioma patient of Henry Ford Hospital
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: brain tumor, Histopathological diagnosis: glioblastoma, grade 4
|
| Biomaterial provider |
Hermelin Brain Tumor Center, Departments Neurology and Neurosurgery at the Henry Ford Hospital (Detroit, MI)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA with high molecular weight was performed using QIamp micro DNA extraction kit (Qiagen, Valencia, CA) following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures using high-resolution microarray scanner 3000, GCOS 1.2
|
| Description |
Snap-frozen sections of areas immediately adjacent to the region used for the histopathologic diagnosis
|
| Data processing |
DAT and CEL files were processed by Affymetrix GCOS 1.2 with default analysis settings. Calls generated by GDAS 3.0 with confidence threshold 0.25. Copy numbers were generated by Affymetrix Chromosome Copy Number Tool, which uses reference set of 145 normal individuals.
|
| |
|
| Submission date |
Oct 23, 2006 |
| Last update date |
Oct 22, 2007 |
| Contact name |
Howard Fine |
| E-mail(s) |
hfine@mail.nih.gov
|
| Phone |
301-402-6383
|
| Fax |
301-480-2246
|
| Organization name |
National Cancer Institute
|
| Department |
Neurological Disorders and Stroke
|
| Street address |
9030 Old Georgetown Rd
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL2004 |
| Series (1) |
| GSE6109 |
High-resolution Global Genomic Survey of 178 Gliomas |
|
