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GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 07, 2007 |
| Title |
kidney_KO2_MT |
| Sample type |
RNA |
| |
|
| Source name |
1/2 kidney, flash-frozen in liquid nitrogen after perfusion with PBS
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6 backcross 10, 4 weeks, homozygous mutant, kidney
|
| Biomaterial provider |
Treier Lab EMBL
|
| Treatment protocol |
QIAGEN RNeasy mini kit, with on-column DNAse treatment, after homogenization with mortar and pestle plus syringe and needle in buffer RLT
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
QIAGEN RNeasy mini kit, with on-column DNAse treatment, after homogenization with mortar and pestle plus syringe and needle in buffer RLT
|
| Label |
biotin-UTP
|
| Label protocol |
CodeLink Gene Expression System: Manual Labelled cRNA Target Preparation
according to manufacturer's instructions
http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/codelink_user_protocols
|
| |
|
| Hybridization protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection
according to manufacturer's instructions
http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/codelink_user_protocols
|
| Scan protocol |
CodeLink Gene Expression System: Single-Assay Bioarray Hybridization and Detection
according to manufacturer's instructions
http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/codelink_user_protocols
|
| Description |
all steps carried out according to manufacturer's protocol
|
| Data processing |
raw values derived from CodeLink Expression Analysis v4.0 software see http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/codelink_software
|
| |
|
| Submission date |
Oct 24, 2006 |
| Last update date |
Jun 07, 2007 |
| Contact name |
Mathias Treier |
| E-mail(s) |
Mathias.Treier@embl-heidelberg.de
|
| Organization name |
EMBL
|
| Department |
Developmental Biology Unit
|
| Lab |
Treier Lab
|
| Street address |
Meyerhofstrasse 1
|
| City |
Heidelberg |
| ZIP/Postal code |
69117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2897 |
| Series (1) |
| GSE6113 |
Kidney Expression Profiles MT |
|
| Data table header descriptions |
| ID_REF |
|
| RAW |
raw expression value from Codelink software (after Background subtraction) |
| VALUE |
median normalized expression value with flagged values removed |
| ROW |
row |
| TYPE_FLAG |
D=discovery (not being a control) |
| GENESPRINGFLAG |
P=present, M=marginal, A=absent |
| CODELINKFLAG |
G=good, I=irregular shape, C=background contaminated, M=MSR masked, L=near background, S=saturated, X=user excluded |
| UNF_VALUE |
median normalized expression value |
| Data table |
| ID_REF |
RAW |
VALUE |
ROW |
TYPE_FLAG |
GENESPRINGFLAG |
CODELINKFLAG |
UNF_VALUE |
| 301077 |
56.44261932 |
4.009008018 |
77 |
D |
P |
G |
4.009008018 |
| 301091 |
349.2526245 |
24.80672564 |
91 |
D |
P |
G |
24.80672564 |
| 383035 |
12.04494476 |
0.855528689 |
35 |
D |
A |
L |
0.855528689 |
| 383049 |
34.07317352 |
2.420150367 |
49 |
D |
P |
G |
2.420150367 |
| 383091 |
19.27777863 |
1.369262625 |
91 |
D |
A |
L |
1.369262625 |
| 383105 |
216.0526428 |
15.34579344 |
105 |
D |
P |
G |
15.34579344 |
| 56001 |
10.71717072 |
0.761219515 |
1 |
D |
A |
L |
0.761219515 |
| 56015 |
134.5844116 |
9.559265529 |
15 |
D |
P |
G |
9.559265529 |
| 56085 |
183.602417 |
13.04091785 |
85 |
D |
P |
G |
13.04091785 |
| 220057 |
137.2571411 |
9.749104238 |
57 |
D |
P |
G |
9.749104238 |
| 302001 |
395.1411743 |
28.0660989 |
1 |
D |
P |
G |
28.0660989 |
| 302029 |
6.416667938 |
0.455763278 |
29 |
D |
A |
L |
0.455763278 |
| 302043 |
171.2467499 |
12.16331916 |
43 |
D |
P |
G |
12.16331916 |
| 302057 |
91.33332825 |
6.487226313 |
57 |
D |
P |
G |
6.487226313 |
| 302071 |
120.155838 |
8.534432381 |
71 |
D |
P |
G |
8.534432381 |
| 302085 |
102.0249939 |
7.246634254 |
85 |
D |
P |
G |
7.246634254 |
| 302099 |
257.0384521 |
18.25693471 |
99 |
D |
P |
G |
18.25693471 |
| 384001 |
76.04225159 |
5.401131272 |
1 |
D |
P |
G |
5.401131272 |
| 384015 |
195.4390259 |
13.88164885 |
15 |
D |
P |
G |
13.88164885 |
| 384029 |
7.033706665 |
0.499590323 |
29 |
D |
A |
L |
0.499590323 |
Total number of rows: 34937
Table truncated, full table size 1737 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM142101.tif.gz |
41.1 Mb |
(ftp)(http) |
TIFF |
| Processed data included within Sample table |
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