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Sample GSM142172 Query DataSets for GSM142172
Status Public on Feb 14, 2007
Title Lung_Feed_BBMP_2-4
Sample type RNA
 
Source name lung tissue, right lobe, feed treatment, 13 weeks, 7 days per week, 2,2-Bis(bromomethyl)-1,3-propanediol, 1250 ppm
Organism Mus musculus
Characteristics Array Type: Affymetrix Mouse 430_2
Amount to Core: 10
Units of Amount: ul
OD260: 0.517
OD280: 0.344
Ratio: 1.83
Dilution: 1:05
RNA Integrity Number: 8.1
Bioanalyzer Results: Good
Sample Preservation: RNA later
Strain or Line: B6C3F1
NCBI Taxonomy ID: 10090
Individual: 2-4
Developmental State: Adult
Sacrifice Method: Pentobarbital (5.2 mg/kg) and exsanguination
Sex: Female
Age: 18
Units of Age: weeks
Biomaterial provider R. Thomas, CIIT Centers for Health Research, 6 Davis Dr, RTP, NC, 27709, R. Thomas, rthomas@ciit.org, 919 558 1311, 919 558 1300
Treatment protocol in vivo Treatment: 2,2-Bis(bromomethyl)-1,3-propanediol, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 7, Units of Treatment Time: days/wk, Vehicle for Chemical: Rodent Chow, Route of Exposure: Diet, Dose or Concentration: 1250, Units of Dose or Concentration: ppm
Extracted molecule total RNA
Extraction protocol Extraction Method: Trizol
Additional Purification Method: Qiagen Rneasy
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Sample Type: tissue, Tissue: Lung, Tissue Harvest Methods: Tissue was excised from animal, minced, placed in RNA later, and stored at -80 degrees Centigrade
Data processing affylmGUI, The Walter and Eliza Hall Institute of Medical Research
 
Submission date Oct 24, 2006
Last update date Aug 28, 2018
Contact name Russell Scott Thomas
E-mail(s) rthomas@thehamner.org
Phone 919-558-1311
Fax 919-558-1300
Organization name The Hamner Institutes for Health Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL1261
Series (1)
GSE6116 Transcriptional Biomarkers to Predict Female Mouse Lung Tumors in Rodent Cancer Bioassays - A 13 Chemical Training Set
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF Affymetrix probe name
VALUE RMA Expressioni Value

Data table
ID_REF VALUE
1442076_at 5.352584933
1425578_a_at 4.231039887
1436026_at 9.199799376
1424560_at 6.428533712
1434336_s_at 7.835703934
1421309_at 6.349075797
1443332_at 4.06143492
1431524_at 3.89007616
1423469_at 3.937672045
1449783_at 3.543577835
1422366_at 3.594237317
1445174_at 2.931407341
1451373_at 4.465293981
1443905_at 7.626947216
1439162_at 3.32917103
1452933_at 7.559267735
1440297_at 3.670200885
1419685_at 6.286103627
1418005_at 11.46277959
1452405_x_at 7.756034354

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM142172.CEL.gz 3.7 Mb (ftp)(http) CEL

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