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Sample GSM142186 Query DataSets for GSM142186
Status Public on Feb 14, 2007
Title Lung_Gavage_CMPH_2-113
Sample type RNA
 
Source name lung tissue, right lobe, gavage treatment, water vehicle, 13 weeks, 5 days per week, 2-chloromethylpyridine hydrochloride, 250 mg per kg
Organism Mus musculus
Characteristics Array Type: Affymetrix Mouse 430_2
Amount to Core: 15
Units of Amount: ul
OD260: 3.98
OD280: 2.138
Ratio: 1.86
Dilution: 1:05
RNA Integrity Number: 7.9
Bioanalyzer Results: Good
Sample Preservation: RNA later
Strain or Line: B6C3F1
NCBI Taxonomy ID: 10090
Individual: 2-113
Developmental State: Adult
Sacrifice Method: Pentobarbital (5.2 mg/kg) and exsanguination
Sex: Female
Age: 18
Units of Age: weeks
Biomaterial provider R. Thomas, CIIT Centers for Health Research, 6 Davis Dr, RTP, NC, 27709, R. Thomas, rthomas@ciit.org, 919 558 1311, 919 558 1300
Treatment protocol in vivo Treatment: 2-Chloromethylpyridine hydrochloride, Treatment Repeat: 1, Units of Treatment Repeat: per day, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 5, Units of Treatment Time: days/wk, Vehicle for Chemical: Distilled water, Amount of Vehicle: 5, Route of Exposure: Gavage, Units of Amount of Vehicle: ml/kg, Dose or Concentration: 250, Units of Dose or Concentration: mg/kg
Extracted molecule total RNA
Extraction protocol Extraction Method: Trizol
Additional Purification Method: Qiagen Rneasy
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Sample Type: tissue, Tissue: Lung, Tissue Harvest Methods: Tissue was excised from animal, minced, placed in RNA later, and stored at -80 degrees Centigrade
Data processing affylmGUI, The Walter and Eliza Hall Institute of Medical Research
 
Submission date Oct 24, 2006
Last update date Aug 28, 2018
Contact name Russell Scott Thomas
E-mail(s) rthomas@thehamner.org
Phone 919-558-1311
Fax 919-558-1300
Organization name The Hamner Institutes for Health Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL1261
Series (1)
GSE6116 Transcriptional Biomarkers to Predict Female Mouse Lung Tumors in Rodent Cancer Bioassays - A 13 Chemical Training Set
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF Affymetrix probe name
VALUE RMA Expressioni Value

Data table
ID_REF VALUE
1446437_at 3.889541046
1432571_at 3.994293784
1447074_at 3.435218587
1444539_at 5.87449618
1436597_at 5.142564748
1430988_at 2.968926739
1439697_at 3.306407424
1417444_at 6.96232848
1448119_at 8.937764128
1442707_at 3.630584032
1440901_at 4.202407692
1435466_at 4.933356002
1429838_at 4.293446807
1437105_at 7.246443014
1450308_a_at 6.259920865
1433202_at 3.741524328
1432047_at 3.637726709
1427599_at 3.584500461
1437619_x_at 4.091816174
1426532_at 7.54607123

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM142186.CEL.gz 3.6 Mb (ftp)(http) CEL

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