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Sample GSM142195 Query DataSets for GSM142195
Status Public on Feb 14, 2007
Title Lung_Gavage_DBET_2-54
Sample type RNA
 
Source name lung tissue, right lobe, gavage treatment, corn oil vehicle, 13 weeks, 5 days per week, 1,2-dibromoethane, 62 mg per kg
Organism Mus musculus
Characteristics Array Type: Affymetrix Mouse 430_2
Amount to Core: 15
Units of Amount: ul
OD260: 5.082
OD280: 2.657
Ratio: 1.88
Dilution: 1:05
RNA Integrity Number: 7.6
Bioanalyzer Results: Good
Sample Preservation: RNA later
Strain or Line: B6C3F1
NCBI Taxonomy ID: 10090
Individual: 2-54
Developmental State: Adult
Sacrifice Method: Pentobarbital (5.2 mg/kg) and exsanguination
Sex: Female
Age: 18
Units of Age: weeks
Biomaterial provider R. Thomas, CIIT Centers for Health Research, 6 Davis Dr, RTP, NC, 27709, R. Thomas, rthomas@ciit.org, 919 558 1311, 919 558 1300
Treatment protocol in vivo Treatment: 1,2-Dibromoethane, Treatment Repeat: 1, Units of Treatment Repeat: per day, Treatment Duration: 13, Units of Duration: weeks, Treatment Time: 5, Units of Treatment Time: days/wk, Vehicle for Chemical: Corn Oil, Amount of Vehicle: 5, Route of Exposure: Gavage, Units of Amount of Vehicle: ml/kg, Dose or Concentration: 62, Units of Dose or Concentration: mg/kg
Extracted molecule total RNA
Extraction protocol Extraction Method: Trizol
Additional Purification Method: Qiagen Rneasy
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Sample Type: tissue, Tissue: Lung, Tissue Harvest Methods: Tissue was excised from animal, minced, placed in RNA later, and stored at -80 degrees Centigrade
Data processing affylmGUI, The Walter and Eliza Hall Institute of Medical Research
 
Submission date Oct 24, 2006
Last update date Aug 28, 2018
Contact name Russell Scott Thomas
E-mail(s) rthomas@thehamner.org
Phone 919-558-1311
Fax 919-558-1300
Organization name The Hamner Institutes for Health Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL1261
Series (1)
GSE6116 Transcriptional Biomarkers to Predict Female Mouse Lung Tumors in Rodent Cancer Bioassays - A 13 Chemical Training Set
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF Affymetrix probe name
VALUE RMA Expressioni Value

Data table
ID_REF VALUE
1421309_at 6.008868534
1443332_at 3.992881011
1431524_at 4.014522289
1423469_at 3.938873208
1449783_at 3.418237047
1422366_at 3.710070158
1445174_at 2.92117532
1451373_at 4.390207966
1443905_at 7.403157508
1439162_at 3.702053196
1452405_x_at 7.587536894
1452933_at 7.128215852
1440297_at 3.519951653
1453510_s_at 4.382420122
1419685_at 6.215354978
1418005_at 11.0905093
1441519_at 4.041411109
1430420_at 3.088654191
1420774_a_at 4.665194271
1429262_at 5.808696849

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM142195.CEL.gz 3.5 Mb (ftp)(http) CEL

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