|
| Status |
Public on Nov 19, 2015 |
| Title |
5719882025G |
| Sample type |
RNA |
| |
|
| Source name |
ST2RX2dox
|
| Organism |
Mus musculus |
| Characteristics |
strain: SKNBE(2)
co-culture conditions: Human neuroblastoma
dox treatment: yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNA mini kit, followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified using illumina Total RNA amplification kit. Total RNA was reverse transcribed using T7 oligo dT primer to generate first strand of cDNA. Biotinylated cRNA was generated from double stranded cDNA templates via in vitro transcription.
|
| |
|
| Hybridization protocol |
Standard Illumina bead array protocol was used using MouseRef-8_V2_R0_11278551 array
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Gene expression signature regulating co-cultured mesenchymal and neuroblastoma cells
|
| Data processing |
Data was bacground corrected using Bead studio Software (Illumina). The data were normalised using quantile normalisation with IlluminaGUI in R. detection scores were calculated in BeadStudio software.
|
| |
|
| Submission date |
Jun 30, 2014 |
| Last update date |
Nov 19, 2015 |
| Contact name |
Vasu Punj |
| E-mail(s) |
vasupunj@gmail.com
|
| Organization name |
USC
|
| Street address |
Biggy Street
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE58541 |
Interaction between bone marrow mesenchymal stromal cell and neuroblastoma cells leads to a VEGFA-mediated increase in osteoblastogenesis |
|
