|
| Status |
Public on Jul 30, 2014 |
| Title |
Old Day 1 #3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse sciatic nerve
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
age: 24 months
tissue: sciatic nerve
time: 1d
treatment: sciatic nerve crush injury
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
M15_inj_o_1d
|
| Data processing |
The data were normalised using standard RMA.
|
| |
|
| Submission date |
Jul 02, 2014 |
| Last update date |
Jul 30, 2014 |
| Contact name |
Michio Painter |
| Organization name |
Children's Hospital Boston
|
| Department |
Neurobiology
|
| Lab |
Clifford Woolf
|
| Street address |
3 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE59040 |
Gene expression from aged and young sciatic nerve after nerve injury |
|
