|
| Status |
Public on Jul 04, 2014 |
| Title |
817 |
| Sample type |
RNA |
| |
|
| Source name |
817
|
| Organism |
Mus musculus |
| Characteristics |
genotype: A-KO
age: P7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol with O/N precipitation in 100% isopropanol
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using the Genisphere FlashTag Biotin HSR kit (Hatfield, PA). One microgram of total RNA was incubated with ATP and Poly A polymerase to add a 3’ polyA tail. A ligation reaction was then performed to covalently attach to the miRNA population a multiple-biotin molecule containing a 3DNA dendrimer
|
| |
|
| Hybridization protocol |
Labeled samples were subsequently processed according to manufacturer’s instructions for the Affymetrix miRNA Array 1.0 (Santa Clara, CA). After hybridization for 16 h at 48oC, the arrays were washed and stained in an Affymetrix Fluidics station 450
|
| Scan protocol |
scanned in an Affymetrix 3000 7G scanner using Command Console (AGCC)
|
| Data processing |
The data were processed using RMA in Partek
|
| |
|
| Submission date |
Jul 03, 2014 |
| Last update date |
Jul 04, 2014 |
| Contact name |
Jie Sun |
| Organization name |
Mayo Clinic
|
| Street address |
200 First Street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE59079 |
Axin2/Runx2 mouse calvaria microRNA expression |
| GSE59083 |
Axin2/Runx2 mouse calvaria expression |
|
