|
Status |
Public on Jul 04, 2014 |
Title |
821 |
Sample type |
RNA |
|
|
Source name |
821
|
Organism |
Mus musculus |
Characteristics |
genotype: A-KO R-Het age: P7
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIzol with O/N precipitation in 100% isopropanol
|
Label |
biotin
|
Label protocol |
Samples were labeled using the Genisphere FlashTag Biotin HSR kit (Hatfield, PA). One microgram of total RNA was incubated with ATP and Poly A polymerase to add a 3’ polyA tail. A ligation reaction was then performed to covalently attach to the miRNA population a multiple-biotin molecule containing a 3DNA dendrimer
|
|
|
Hybridization protocol |
Labeled samples were subsequently processed according to manufacturer’s instructions for the Affymetrix miRNA Array 1.0 (Santa Clara, CA). After hybridization for 16 h at 48oC, the arrays were washed and stained in an Affymetrix Fluidics station 450
|
Scan protocol |
scanned in an Affymetrix 3000 7G scanner using Command Console (AGCC)
|
Data processing |
The data were processed using RMA in Partek
|
|
|
Submission date |
Jul 03, 2014 |
Last update date |
Jul 04, 2014 |
Contact name |
Jie Sun |
Organization name |
Mayo Clinic
|
Street address |
200 First Street SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55905 |
Country |
USA |
|
|
Platform ID |
GPL8786 |
Series (2) |
GSE59079 |
Axin2/Runx2 mouse calvaria microRNA expression |
GSE59083 |
Axin2/Runx2 mouse calvaria expression |
|