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Sample GSM1426307 Query DataSets for GSM1426307
Status Public on Jul 04, 2014
Title 590
Sample type RNA
 
Source name 590
Organism Mus musculus
Characteristics Sex: M
genotype: WT
age: P7
ChIP: 1
chip position: F
Extracted molecule total RNA
Extraction protocol TRIzol with O/N precipitation in 100% isopropanol
Label Cy3
Label protocol Labeling of high quality samples was performed according to manufacturer’s instructions for the Illumina Total Prep RNA Amplification Kit (Life Technologies, Grand Island, NY). Briefly, 200 ng of total RNA was reverse transcribed with T7 Oligo d(T) to create second strand cDNA. Subsequently, the products were column purified and then in vitro transcribed to generate biotin-labeled cRNA.
 
Hybridization protocol cRNA products were column purified and hybridized, per Illuminas protocol, onto Illumina Mouse Ref8 Beadchips for 16 hours at 58° C. Following hybridization, the arrays were washed, stained with streptavidin-cy3 conjugate
Scan protocol scanned in an Illumina BeadArray Reader
Data processing The data were normalized using fastlo normalization in R
 
Submission date Jul 03, 2014
Last update date Jul 04, 2014
Contact name Jie Sun
Organization name Mayo Clinic
Street address 200 First Street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL6885
Series (2)
GSE59081 Axin2/Runx2 mouse calvaria gene expression
GSE59083 Axin2/Runx2 mouse calvaria expression

Data table header descriptions
ID_REF
VALUE fastlo signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 2386.292563 0
ILMN_2721178 504.4258993 0
ILMN_3033922 984.1030835 0
ILMN_3092673 3873.338687 0
ILMN_2816356 111.7113729 0.01253133
ILMN_2808939 501.47787 0
ILMN_2634564 348.1206242 0
ILMN_2737647 75.3606053 0.2957394
ILMN_2734484 457.55509 0
ILMN_2952292 91.86766094 0.06390978
ILMN_2699078 64.56627972 0.6177945
ILMN_1213681 253.8165375 0.001253133
ILMN_2735413 60.73711797 0.7218045
ILMN_2735415 96.08677933 0.05639098
ILMN_2891688 708.4323305 0
ILMN_2637698 807.2165916 0
ILMN_2674228 342.1649956 0
ILMN_2601546 74.32796914 0.2756892
ILMN_1230831 68.25867168 0.4949875
ILMN_2848071 100.0300544 0.03884712

Total number of rows: 25697

Table truncated, full table size 838 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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