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Sample GSM1426309 Query DataSets for GSM1426309
Status Public on Jul 04, 2014
Title 817
Sample type RNA
 
Source name 817
Organism Mus musculus
Characteristics Sex: M
genotype: A-KO
age: P7
ChIP: 1
chip position: H
Extracted molecule total RNA
Extraction protocol TRIzol with O/N precipitation in 100% isopropanol
Label Cy3
Label protocol Labeling of high quality samples was performed according to manufacturer’s instructions for the Illumina Total Prep RNA Amplification Kit (Life Technologies, Grand Island, NY). Briefly, 200 ng of total RNA was reverse transcribed with T7 Oligo d(T) to create second strand cDNA. Subsequently, the products were column purified and then in vitro transcribed to generate biotin-labeled cRNA.
 
Hybridization protocol cRNA products were column purified and hybridized, per Illuminas protocol, onto Illumina Mouse Ref8 Beadchips for 16 hours at 58° C. Following hybridization, the arrays were washed, stained with streptavidin-cy3 conjugate
Scan protocol scanned in an Illumina BeadArray Reader
Description damaged during processing; did not scan well; excluded from analyses
Data processing The data were normalized using fastlo normalization in R
 
Submission date Jul 03, 2014
Last update date Jul 04, 2014
Contact name Jie Sun
Organization name Mayo Clinic
Street address 200 First Street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL6885
Series (2)
GSE59081 Axin2/Runx2 mouse calvaria gene expression
GSE59083 Axin2/Runx2 mouse calvaria expression

Data table header descriptions
ID_REF
VALUE fastlo signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 4721.646798 0
ILMN_2721178 626.1564568 0
ILMN_3033922 1135.229216 0
ILMN_3092673 6210.073616 0
ILMN_2816356 98.96106759 0.1140351
ILMN_2808939 582.4054395 0
ILMN_2634564 309.8269726 0
ILMN_2737647 74.26455891 0.3596491
ILMN_2734484 424.7172997 0
ILMN_2952292 112.6066663 0.04260652
ILMN_2699078 65.38647297 0.5626566
ILMN_1213681 270.1227517 0
ILMN_2735413 73.61900719 0.3947369
ILMN_2735415 73.21353872 0.4761905
ILMN_2891688 405.6567846 0
ILMN_2637698 990.5131536 0
ILMN_2674228 304.2483305 0
ILMN_2601546 68.57869589 0.5839599
ILMN_1230831 60.19203096 0.7280702
ILMN_2848071 63.65721836 0.7142857

Total number of rows: 25697

Table truncated, full table size 827 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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