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Sample GSM1426314 Query DataSets for GSM1426314
Status Public on Jul 04, 2014
Title 844
Sample type RNA
Source name 844
Organism Mus musculus
Characteristics Sex: M
genotype: WT
age: P7
ChIP: 2
chip position: E
Extracted molecule total RNA
Extraction protocol TRIzol with O/N precipitation in 100% isopropanol
Label Cy3
Label protocol Labeling of high quality samples was performed according to manufacturer’s instructions for the Illumina Total Prep RNA Amplification Kit (Life Technologies, Grand Island, NY). Briefly, 200 ng of total RNA was reverse transcribed with T7 Oligo d(T) to create second strand cDNA. Subsequently, the products were column purified and then in vitro transcribed to generate biotin-labeled cRNA.
Hybridization protocol cRNA products were column purified and hybridized, per Illuminas protocol, onto Illumina Mouse Ref8 Beadchips for 16 hours at 58° C. Following hybridization, the arrays were washed, stained with streptavidin-cy3 conjugate
Scan protocol scanned in an Illumina BeadArray Reader
Data processing The data were normalized using fastlo normalization in R
Submission date Jul 03, 2014
Last update date Jul 04, 2014
Contact name Jie Sun
Organization name Mayo Clinic
Street address 200 First Street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
Platform ID GPL6885
Series (2)
GSE59081 Axin2/Runx2 mouse calvaria gene expression
GSE59083 Axin2/Runx2 mouse calvaria expression

Data table header descriptions
VALUE fastlo signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 2137.120615 0
ILMN_2721178 411.4800402 0
ILMN_3033922 976.8202384 0
ILMN_3092673 4234.253797 0
ILMN_2816356 109.520178 0.01253133
ILMN_2808939 473.2932171 0
ILMN_2634564 323.0933728 0
ILMN_2737647 76.49012128 0.2055138
ILMN_2734484 389.981002 0
ILMN_2952292 111.9218542 0.01002506
ILMN_2699078 73.19345396 0.2706767
ILMN_1213681 253.6311375 0.001253133
ILMN_2735413 67.71735167 0.4912281
ILMN_2735415 74.71932834 0.2531328
ILMN_2891688 729.8399885 0
ILMN_2637698 901.1260002 0
ILMN_2674228 318.8485326 0
ILMN_2601546 73.35337957 0.2969925
ILMN_1230831 62.81489659 0.6929824
ILMN_2848071 83.69989143 0.1203008

Total number of rows: 25697

Table truncated, full table size 837 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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