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Sample GSM1429429 Query DataSets for GSM1429429
Status Public on Aug 01, 2014
Title epidermis_wildtype_1
Sample type RNA
 
Source name Epidermis from posterior ear lobe
Organism Mus musculus
Characteristics treatment status: untreated
phenotype: wildtype
strain: B6D2
Treatment protocol Seven wk old B6D2 females in the telogen stage of the hair cycle were shaved, and treated 3 days later. Glucocorticoid FA was applied topically (2 ug/animal) in 200 ul acetone to the back skin once or up to four applications every third day as described previously (Chebotaev et al., 2007b). Control animals were treated with acetone only.
Extracted molecule total RNA
Extraction protocol Total RNA from murine epidermis was isolated with RiboPure kit (Ambion). Total RNA from murine s.c. adipose was isolated with RNeasy Lipid Tissue Kit (Qiagen). The RNA samples were treated with TURBOTM DNase (Ambion). Purity of RNA samples isolated from epidermis and s.c. fat was confirmed by the expression of adipose and keratinocyte cell markers.
Label biotin
Label protocol RNA amplification, labeling, hybridization with the Mouse Whole-Genome Gene Expression BeadChips (Illumina), and data analysis were performed at the Genomics Core Facility at the Center for Genetic Medicine at Northwestern University according to the DNA chip manufacturer's protocols.
 
Hybridization protocol RNA amplification, labeling, hybridization with the Mouse Whole-Genome Gene Expression BeadChips (Illumina), and data analysis were performed at the Genomics Core Facility at the Center for Genetic Medicine at Northwestern University according to the DNA chip manufacturer's protocols.
Scan protocol RNA amplification, labeling, hybridization with the Mouse Whole-Genome Gene Expression BeadChips (Illumina), and data analysis were performed at the Genomics Core Facility at the Center for Genetic Medicine at Northwestern University according to the DNA chip manufacturer's protocols.
Data processing The data underwent background subtraction and quantile normalization using the neqc function from limma package in R
 
Submission date Jul 07, 2014
Last update date Aug 01, 2014
Contact name Ben Readhead
E-mail(s) ben.readhead@asu.edu
Organization name ASU-Banner Neurodegenerative Disease Research Center
Street address 797 E. Tyler Street
City Tempe
State/province AZ
ZIP/Postal code 85281
Country USA
 
Platform ID GPL6885
Series (1)
GSE59151 REDD1 (regulated in development and DNA damage response 1) dissociates therapeutic and adverse effects of topical steroids in skin

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 9.407366476 0
ILMN_2721178 7.041146424 0
ILMN_3033922 7.493824714 0
ILMN_3092673 10.88923683 0
ILMN_2816356 4.719342057 0.03132832
ILMN_2808939 8.986157289 0
ILMN_2634564 7.823209649 0
ILMN_2737647 4.267985706 0.5275689
ILMN_2734484 6.889208518 0
ILMN_2952292 6.3510211 0
ILMN_2699078 4.295683851 0.4260652
ILMN_1213681 6.104865724 0
ILMN_2735413 4.316387338 0.368421
ILMN_2735415 4.504640596 0.1002506
ILMN_2891688 7.100155572 0
ILMN_2637698 8.986752077 0
ILMN_2674228 5.979679212 0
ILMN_2601546 4.618700318 0.05137845
ILMN_1230831 4.363510372 0.2481203
ILMN_2848071 4.542742664 0.07518797

Total number of rows: 25697

Table truncated, full table size 804 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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