|
Status |
Public on Aug 01, 2014 |
Title |
epidermis_REDD1_KO_1 |
Sample type |
RNA |
|
|
Source name |
Epidermis from posterior ear lobe
|
Organism |
Mus musculus |
Characteristics |
treatment status: untreated phenotype: REDD1_KO strain: B6D2
|
Treatment protocol |
Seven wk old B6D2 females in the telogen stage of the hair cycle were shaved, and treated 3 days later. Glucocorticoid FA was applied topically (2 ug/animal) in 200 ul acetone to the back skin once or up to four applications every third day as described previously (Chebotaev et al., 2007b). Control animals were treated with acetone only.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from murine epidermis was isolated with RiboPure kit (Ambion). Total RNA from murine s.c. adipose was isolated with RNeasy Lipid Tissue Kit (Qiagen). The RNA samples were treated with TURBOTM DNase (Ambion). Purity of RNA samples isolated from epidermis and s.c. fat was confirmed by the expression of adipose and keratinocyte cell markers.
|
Label |
biotin
|
Label protocol |
RNA amplification, labeling, hybridization with the Mouse Whole-Genome Gene Expression BeadChips (Illumina), and data analysis were performed at the Genomics Core Facility at the Center for Genetic Medicine at Northwestern University according to the DNA chip manufacturer's protocols.
|
|
|
Hybridization protocol |
RNA amplification, labeling, hybridization with the Mouse Whole-Genome Gene Expression BeadChips (Illumina), and data analysis were performed at the Genomics Core Facility at the Center for Genetic Medicine at Northwestern University according to the DNA chip manufacturer's protocols.
|
Scan protocol |
RNA amplification, labeling, hybridization with the Mouse Whole-Genome Gene Expression BeadChips (Illumina), and data analysis were performed at the Genomics Core Facility at the Center for Genetic Medicine at Northwestern University according to the DNA chip manufacturer's protocols.
|
Data processing |
The data underwent background subtraction and quantile normalization using the neqc function from limma package in R
|
|
|
Submission date |
Jul 07, 2014 |
Last update date |
Aug 01, 2014 |
Contact name |
Ben Readhead |
E-mail(s) |
ben.readhead@asu.edu
|
Organization name |
ASU-Banner Neurodegenerative Disease Research Center
|
Street address |
797 E. Tyler Street
|
City |
Tempe |
State/province |
AZ |
ZIP/Postal code |
85281 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE59151 |
REDD1 (regulated in development and DNA damage response 1) dissociates therapeutic and adverse effects of topical steroids in skin |
|