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Status |
Public on Aug 31, 2014 |
Title |
Infected_Non PZQ_6weeks_rep1 |
Sample type |
RNA |
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Source name |
Infected liver, non PZQ
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: Female age: 6 weeks tissue: Liver infection: S. japonicum treatment: Non PZQ
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Treatment protocol |
Mice were euthanised and whole liver from each experimental grouping were stored in Trizol reagent at -80C for RNA extraction.
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Growth protocol |
The complete life cycle of schistosoma japonicum was maintained in our laboratory. The cercarial of S. japonicum were shed from Oncomelania hupensis snail to infect the experimental mice.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of liver tissues were extracted by a combination use of Trizol (Invitrogen) and RNeasy Mini kit (Qiagen) method.
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Label |
Cy3
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Label protocol |
cRNA was synthesised from total RNA using Illumina Total Prep RNA Amplication kit (Ambion).
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Hybridization protocol |
Synthesized cRNA was hybridised to Illumina mouse Ref-8 v2 expression beadchips according to manufacturer instructions.
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Scan protocol |
Images on the arrays were scanned using Beadstation 500 at green factor level 2.
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Description |
Gene expression in the non PZQ-treated liver of infected C57BL/6 mice at 6 weeks post infection 9254035024_A_6 weeks p.i. (1)
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Data processing |
Feature extraction of the data was generated using GenomeStudio version 1.1.1 and analysed further using GeneSpring GX 11. Non-normalised (Raw data) represent relative signal intensity; normalised data were normalised to the median of all samples and are presented in log2 scale.
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Submission date |
Jul 10, 2014 |
Last update date |
Aug 31, 2014 |
Contact name |
Geoffrey Gobert |
E-mail(s) |
geoffG@qimr.edu.au
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Organization name |
QIMR
|
Street address |
300 Herston Rd
|
City |
Brisbane |
ZIP/Postal code |
4006 |
Country |
Australia |
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|
Platform ID |
GPL6885 |
Series (1) |
GSE59276 |
Characterising Host Gene Expression during the Recovery from Hepatic Schistosomiasis Japonica |
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