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Sample GSM1432657 Query DataSets for GSM1432657
Status Public on Aug 31, 2014
Title Uninfected_PZQ_10weeks_rep3
Sample type RNA
 
Source name Uninfected liver, PZQ
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Female
age: 10 weeks
tissue: Liver
infection: none
treatment: PZQ
Treatment protocol Mice were euthanised and whole liver from each experimental grouping were stored in Trizol reagent at -80C for RNA extraction.
Growth protocol The complete life cycle of schistosoma japonicum was maintained in our laboratory. The cercarial of S. japonicum were shed from Oncomelania hupensis snail to infect the experimental mice.
Extracted molecule total RNA
Extraction protocol Total RNA of liver tissues were extracted by a combination use of Trizol (Invitrogen) and RNeasy Mini kit (Qiagen) method.
Label Cy3
Label protocol cRNA was synthesised from total RNA using Illumina Total Prep RNA Amplication kit (Ambion).
 
Hybridization protocol Synthesized cRNA was hybridised to Illumina mouse Ref-8 v2 expression beadchips according to manufacturer instructions.
Scan protocol Images on the arrays were scanned using Beadstation 500 at green factor level 2.
Description Gene expression in the PZQ-treated, uninfected liver of C57BL/6 mice at 10 weeks
9254035017_B_10 weeks, Control (3)
Data processing Feature extraction of the data was generated using GenomeStudio version 1.1.1 and analysed further using GeneSpring GX 11.
Non-normalised (Raw data) represent relative signal intensity; normalised data were normalised to the median of all samples and are presented in log2 scale.
 
Submission date Jul 10, 2014
Last update date Aug 31, 2014
Contact name Geoffrey Gobert
E-mail(s) geoffG@qimr.edu.au
Organization name QIMR
Street address 300 Herston Rd
City Brisbane
ZIP/Postal code 4006
Country Australia
 
Platform ID GPL6885
Series (1)
GSE59276 Characterising Host Gene Expression during the Recovery from Hepatic Schistosomiasis Japonica

Data table header descriptions
ID_REF
VALUE normalised to the median of all samples and are presented in log2 scale
Detection Pval_9254035017_B_10 weeks, Control (3)

Data table
ID_REF VALUE Detection Pval_9254035017_B_10 weeks, Control (3)
ILMN_2749327 -0.20637941 0.50251
ILMN_2877059 -0.1297059 0.99875
ILMN_3151270 -0.089995384 0.24812
ILMN_2627041 -0.28691888 0.94737
ILMN_1233116 -0.004370689 0.97494
ILMN_2659804 0.001755476 0.73684
ILMN_2711615 -0.18666601 0.74812
ILMN_2588946 -0.028040886 0.74561
ILMN_2713507 0.20260143 0.95739
ILMN_2906473 -0.001463175 1
ILMN_2787209 -0.04509878 0.1792
ILMN_1220236 -0.4075811 0.12531
ILMN_2841043 -9.48E-04 0.99749
ILMN_2684552 -0.08514857 0.8584
ILMN_2651369 0.1703775 0.94737
ILMN_2493747 -0.07574129 1
ILMN_2782248 -0.27807808 0.00752
ILMN_2724226 0.35465384 1
ILMN_2776561 0.07781887 0.84712
ILMN_1251446 -0.009972096 0.31955

Total number of rows: 25697

Table truncated, full table size 770 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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