|
| Status |
Public on Aug 31, 2014 |
| Title |
Infected_Non PZQ_14weeks_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Infected liver, non PZQ
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Female
age: 14 weeks
tissue: Liver
infection: S. japonicum
treatment: Non PZQ
|
| Treatment protocol |
Mice were euthanised and whole liver from each experimental grouping were stored in Trizol reagent at -80C for RNA extraction.
|
| Growth protocol |
The complete life cycle of schistosoma japonicum was maintained in our laboratory. The cercarial of S. japonicum were shed from Oncomelania hupensis snail to infect the experimental mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of liver tissues were extracted by a combination use of Trizol (Invitrogen) and RNeasy Mini kit (Qiagen) method.
|
| Label |
Cy3
|
| Label protocol |
cRNA was synthesised from total RNA using Illumina Total Prep RNA Amplication kit (Ambion).
|
| |
|
| Hybridization protocol |
Synthesized cRNA was hybridised to Illumina mouse Ref-8 v2 expression beadchips according to manufacturer instructions.
|
| Scan protocol |
Images on the arrays were scanned using Beadstation 500 at green factor level 2.
|
| Description |
Gene expression in the non PZQ-treated liver of infected C57BL/6 mice at 14 weeks post infection
9254035003_D_14 weeks, non PZQ (2)
|
| Data processing |
Feature extraction of the data was generated using GenomeStudio version 1.1.1 and analysed further using GeneSpring GX 11.
Non-normalised (Raw data) represent relative signal intensity; normalised data were normalised to the median of all samples and are presented in log2 scale.
|
| |
|
| Submission date |
Jul 10, 2014 |
| Last update date |
Aug 31, 2014 |
| Contact name |
Geoffrey Gobert |
| E-mail(s) |
geoffG@qimr.edu.au
|
| Organization name |
QIMR
|
| Street address |
300 Herston Rd
|
| City |
Brisbane |
| ZIP/Postal code |
4006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE59276 |
Characterising Host Gene Expression during the Recovery from Hepatic Schistosomiasis Japonica |
|
