|
| Status |
Public on Sep 01, 2014 |
| Title |
MCF7-B, estradiol treated, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cells chronically cultured in BPA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: MCF7
treatment: estradiol treated
|
| Treatment protocol |
Cells were treated with either ethanol of 10 nM estradiol for 3 hours.
|
| Growth protocol |
Cells were grown in phenol-red free DMEM containing 5% charcoal/dextran treadted FBS for 72 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Total RNA isolation Nucleospin® RNA II kit (Macherey-Nagel
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit
|
| |
|
| Hybridization protocol |
Samples were hybridized using Herring sperm DNA, acetylated BSA , BioArray Eukaryotic Hybridization Controls (ENZO Life Sciences). 65 C for 5 minutes, 99 C for 5 min, 45 C for 5 minutes. Centrifuge top speed for 5 minutes. Hybridize 16 h at 45C at 60 rpm. Fluidics washing is FS450_001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G using AGCC
|
| Data processing |
Data was normalized using the Expression Console Software
|
| |
|
| Submission date |
Jul 11, 2014 |
| Last update date |
Sep 01, 2014 |
| Contact name |
Craig Joseph Burd |
| E-mail(s) |
craig.burd@osumc.edu
|
| Phone |
614-688-7458
|
| Organization name |
The Ohio State University
|
| Department |
Molecular Genetics
|
| Street address |
460 W. 12th Ave. Rm 920 BRT
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE59345 |
Chronic Bisphenol A and Genistein Exposure in MCF7 cells |
|
