NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1435590 Query DataSets for GSM1435590
Status Public on Jul 16, 2014
Title T44L2DD
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics experiment time: 44
circadian time: 0
light regimen ld vs dd: DD
health state: Basal
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 11, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59396 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #1
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 10.8486198364612 2195.3 530.976 0 36
ILMN_2721178 8.79582024442341 651.7 116.655 0 25
ILMN_3033922 8.8865046007691 683.5 166.202460631604 0 45
ILMN_3092673 11.5353720730779 3435.2 905.112 0 36
ILMN_2816356 2.2338516123445 152.6 37.2021756353039 0.15789 37
ILMN_2808939 7.76118671365001 400.8 110.456344100282 0 48
ILMN_2634564 6.64755044926759 272.1 62.255 0 25
ILMN_2737647 3.72979059847585 172.5 46.9041818711296 0.00251 37
ILMN_2734484 7.46950029768814 357 98.6297880155889 0 44
ILMN_2952292 3.51567215171375 169.3 27.8955489639476 0.00376 37
ILMN_2699078 0.379043549576677 134.9 19.0663391347159 0.80702 42
ILMN_1213681 7.96653282116335 437.4 88.0266050691494 0 47
ILMN_2735413 1.54855823573208 145.2 29.8727502583877 0.42481 38
ILMN_2735415 2.48678384813882 155.6 35.7087494040326 0.08647 34
ILMN_2891688 8.4246236500214 540.5 203.913894465286 0 41
ILMN_2637698 9.56253160359657 995.5 329.828970953129 0 45
ILMN_2674228 7.37021499076025 344 107.117448158552 0 37
ILMN_2601546 1.10736584946438 141 26.6848764471564 0.59273 39
ILMN_1230831 1.28080064696939 142.6 25.4576339238351 0.52882 45
ILMN_2848071 1.96785041459965 149.6 35.624631619148 0.23935 42

Total number of rows: 25697

Table truncated, full table size 1521 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap