|
Status |
Public on Nov 18, 2006 |
Title |
JS_8 |
Sample type |
RNA |
|
|
Source name |
bladder
|
Organism |
Mus musculus |
Characteristics |
gene reported: SMGA/EGFP transgenic / PMID: 14713859 strain: FVB/N sex: Unknown developmental stage: E14 theiler stage: 22 somite count: ND developmental landmark: Individual fingers visible in the anterior footplate with indentations between the toes which are not discernable
|
Treatment protocol |
pool size: N/A Pooled sample: no Dissection Method: Bladder region microdissected based on SMGA/EGFP expression. Female FVB/N mice were time-mated to SMGA/EGFP transgenic males. Pregnant FVB/N mice were euthanized by standard carbon dioxide asphyxiation at 14 days post-coitus. Embryonic samples were euthanized by decapitation and tissues were micro-dissected, rapidly frozen and stored at -80 °C until RNA was extracted for gene expression analysis.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen: RNeasy
|
Label |
Biotin
|
Label protocol |
Biotin-X-X-NHS (EPICENTRE Biotechnologies)
|
|
|
Hybridization protocol |
Affymetrix standard protocol Amount labeled target hybridization to array: 10 ug
|
Scan protocol |
Affymetrix standard protocol
|
Description |
>> Amplification protocol << Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies) Target Amplified protocol: Potter protocols Rounds of amplification: 2 URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:6962
|
Data processing |
Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
|
|
|
Submission date |
Nov 03, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
GUDMAP Developers |
E-mail(s) |
gudmap-db@gudmap.org
|
Phone |
+44 131 651 8500
|
Organization name |
IGMM MRC Human Genetics Unit
|
Lab |
GUDMAP Database Group
|
Street address |
Crewe Road
|
City |
Edinburgh |
ZIP/Postal code |
EH4 2XU |
Country |
United Kingdom |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE6223 |
Gene expression profiles of E14 bladder, urogenital sinus and urethra isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 6) |
|
Relations |
Reanalyzed by |
GSE119085 |