|
| Status |
Public on Jul 16, 2014 |
| Title |
T20L4MR |
| Sample type |
RNA |
| |
|
| Source name |
Mouse lung tissue
|
| Organism |
Mus musculus |
| Characteristics |
experiment time: 20
circadian time: 0
light regimen ld vs dd: LD
health state: Basal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
|
| Scan protocol |
The chips were read using standard illumina protocols using an Illumina iScan reader.
|
| Data processing |
VSN normalized, background corrected
|
| |
|
| Submission date |
Jul 11, 2014 |
| Last update date |
Jul 16, 2014 |
| Contact name |
Jen-hwa Chu |
| E-mail(s) |
jen-hwa.chu@yale.edu
|
| Organization name |
Yale University School of Medicine
|
| Department |
Pulmonary, Critical Care and Sleep Medicine
|
| Street address |
PO Box 208057
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE59396 |
CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #1 |
| GSE59444 |
CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION |
|
