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Sample GSM1435619 Query DataSets for GSM1435619
Status Public on Jul 16, 2014
Title T44L2MR
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics experiment time: 44
circadian time: 0
light regimen ld vs dd: LD
health state: Basal
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 11, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59396 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #1
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 11.3377822492491 3568.2 793.32148052098 0 41
ILMN_2721178 9.45255746547117 1107.9 224.058557803535 0 37
ILMN_3033922 9.16158153076611 941.1 212.403284823941 0 44
ILMN_3092673 11.6726599305341 4449.6 1128.32967151981 0 53
ILMN_2816356 2.98033717521486 195.3 56.8795626741275 0.03383 26
ILMN_2808939 7.99979497019197 528.3 149.719961421315 0 32
ILMN_2634564 6.97810203236115 359 96.8679932949992 0 37
ILMN_2737647 0.692269771651382 166.1 38.4827766669714 0.72556 41
ILMN_2734484 7.72217404695084 469.9 185.379833223574 0 54
ILMN_2952292 4.74436601127563 229.2 61.9091080859674 0 46
ILMN_2699078 2.50088597605036 188.1 47.1559798859063 0.10025 39
ILMN_1213681 8.08093062034203 547.6 152.467341411202 0 53
ILMN_2735413 1.91708953999335 180.1 40.7111625970077 0.2619 40
ILMN_2735415 2.65819984303543 190.4 45.5297811767199 0.06642 46
ILMN_2891688 8.25043828485585 591.6 193.83 0 36
ILMN_2637698 9.7093337691504 1285.8 290.73348352056 0 40
ILMN_2674228 7.62234631881197 451.5 134.866631714446 0 46
ILMN_2601546 2.28194327852187 185 52.0457375776345 0.15163 33
ILMN_1230831 1.30977187581113 172.7 38.64 0.52882 36
ILMN_2848071 0.849374928090987 167.7 36.3949550350045 0.68922 28

Total number of rows: 25697

Table truncated, full table size 1519 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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