|
| Status |
Public on Nov 18, 2006 |
| Title |
060301_2UGS |
| Sample type |
RNA |
| |
|
| Source name |
urogenital sinus
|
| Organism |
Mus musculus |
| Characteristics |
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: Unknown
developmental stage: E14
theiler stage: 21
somite count: ND
developmental landmark: Distal borders of anterior and posterior footplates indented; digit widths and locations discernable
|
| Treatment protocol |
pool size: N/A
Pooled sample: no
Dissection Method: Urogenital sinus region microdissected based on an absence of SMGA/EGFP expression between the developing bladder and urethra regions.
Female FVB/N mice were time-mated to SMGA/EGFP transgenic males. Pregnant FVB/N mice were euthanized by standard carbon dioxide asphyxiation at 14 days post-coitus. Embryonic samples were euthanized by decapitation and tissues were micro-dissected, rapidly frozen and stored at -80 °C until RNA was extracted for gene expression analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen: RNeasy
|
| Label |
Biotin
|
| Label protocol |
Biotin-X-X-NHS (EPICENTRE Biotechnologies)
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
Amount labeled target hybridization to array: 10 ug
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:6964
|
| Data processing |
Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
|
| |
|
| Submission date |
Nov 03, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6223 |
Gene expression profiles of E14 bladder, urogenital sinus and urethra isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 6) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
