NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1435620 Query DataSets for GSM1435620
Status Public on Jul 16, 2014
Title RNACtrl_CHP5
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics experiment time: NA
circadian time: NA
light regimen ld vs dd: NA
health state: NA
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 11, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59396 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #1
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 10.9939960232722 2898.7 519.724807874321 0 45
ILMN_2721178 8.6360879749427 733 218.707698312154 0 29
ILMN_3033922 8.42640199166745 662 191.090669678035 0 38
ILMN_3092673 10.8476154840956 2639.1 456.004895693018 0 41
ILMN_2816356 2.21490841793652 195.1 51.1901502244328 0.21805 30
ILMN_2808939 8.75197572323848 776.9 192.551635796739 0 38
ILMN_2634564 7.50574538398276 447.9 138.571413202724 0 37
ILMN_2737647 -0.53759139455168 163.4 40.2061939631197 0.94862 33
ILMN_2734484 7.02874747822178 380.4 89.6344562710122 0 39
ILMN_2952292 4.25131787794669 231.1 51.7628563354071 0.00125 41
ILMN_2699078 1.82809543445714 189.6 36.9642809614904 0.34336 43
ILMN_1213681 6.95574428555418 371.9 113.084638200774 0 37
ILMN_2735413 0.491321059745524 173.7 44.8739334135086 0.79699 44
ILMN_2735415 0.773980878726776 176.7 46.1316710297817 0.7218 37
ILMN_2891688 9.21309589921107 991.1 279.030641430292 0 33
ILMN_2637698 9.65322367452162 1270.4 345.884166136584 0 44
ILMN_2674228 8.5831784789899 714.1 195.108284683147 0 28
ILMN_2601546 1.46367647847119 184.8 45.4136254443532 0.48246 39
ILMN_1230831 -0.479043561116431 164 38.478 0.94862 36
ILMN_2848071 1.90063874041804 190.6 44.934 0.32206 36

Total number of rows: 25697

Table truncated, full table size 1529 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap