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Sample GSM1435627 Query DataSets for GSM1435627
Status Public on Jul 16, 2014
Title RNACtrl_CHP6
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics experiment time: NA
circadian time: NA
light regimen ld vs dd: NA
health state: NA
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 11, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59396 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #1
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 11.1842582367458 2487.6 504.11120928323 0 53
ILMN_2721178 8.57457347793022 585.5 178.185102407581 0 30
ILMN_3033922 8.33464967569895 528.4 154.386113319819 0 34
ILMN_3092673 10.8720151563479 2044.9 463.434442276359 0 48
ILMN_2816356 3.14253049475997 206.2 53.3336148315488 0.05013 31
ILMN_2808939 8.47273853858041 560.1 142.405956458991 0 35
ILMN_2634564 7.38845560085212 376.6 132.489183377361 0 32
ILMN_2737647 2.0610149712947 190.1 51.1783086180073 0.36842 33
ILMN_2734484 6.61909757444829 308.9 100.036022306967 0 31
ILMN_2952292 5.06580013491157 244.5 61.5432896098348 0.00125 44
ILMN_2699078 1.09190524538299 178.9 41.7166582434403 0.71554 47
ILMN_1213681 6.82575647796712 323.7 83.324173551257 0 58
ILMN_2735413 1.37909463347495 181.9 40.1428471337049 0.63033 39
ILMN_2735415 1.40651806534003 182.2 35.164450187654 0.61153 37
ILMN_2891688 8.90091491002331 680.1 243.979130255028 0 35
ILMN_2637698 9.85098684417908 1115.6 312.58645211845 0 32
ILMN_2674228 8.54287244718684 577.4 181.92301697971 0 43
ILMN_2601546 1.82617669026958 187.1 38.0936377233784 0.44862 35
ILMN_1230831 1.09190524538299 178.9 45.9680289005304 0.71554 41
ILMN_2848071 2.45883085392775 195.6 42.5799516204516 0.21053 30

Total number of rows: 25697

Table truncated, full table size 1536 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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