|
| Status |
Public on Feb 24, 2015 |
| Title |
MiaPaCa2 rep-3 |
| Sample type |
RNA |
| |
|
| Source name |
MiaPaCa2 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MiaPaCa2
cell type: dasatinib-resistant pancreatic cancer cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
5957936057_C
|
| Data processing |
The data were normalised using quantile normalisation and log2 transformed with 'beadarray' Bioconductor package in R
|
| |
|
| Submission date |
Jul 11, 2014 |
| Last update date |
Feb 24, 2015 |
| Contact name |
Phillip H Koeffler |
| E-mail(s) |
phillip_koeffler@nuhs.edu.sg
|
| Organization name |
Cancer Science Institute
|
| Lab |
H. Phillip Koeffler's lab
|
| Street address |
14 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE59357 |
Gene expression profiles of dasatinib-resistant and dasatinib-sensitive pancreatic cancer cell lines |
|
