|
Status |
Public on Jul 12, 2014 |
Title |
Testis_Wt(TgMsi1)_rep1 |
Sample type |
RNA |
|
|
Source name |
Mouse testis
|
Organism |
Mus musculus |
Characteristics |
strain: B6xCBA age: 3 months tissue: testis genotype/variation: Wt(TgMsi1)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from samples using a QIAGEN RNeasy Mini Kit (Hilden, Germany). The quality and quantity of mouse total RNA was ascertained on the Agilent Bioanalyser 2100.
|
Label |
Cy3
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
comparison 1
|
Data processing |
GenomeStudio was used to convert the signal on the array into a TXT file for analysis
|
|
|
Submission date |
Jul 11, 2014 |
Last update date |
Jul 12, 2014 |
Contact name |
Jessie M Sutherland |
E-mail(s) |
jessie.sutherland@newcastle.edu.au
|
Organization name |
University of Newcastle
|
Street address |
University Drive
|
City |
Callaghan |
ZIP/Postal code |
2304 |
Country |
Australia |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE59358 |
Microarray analysis of differential gene expression of whole testis extracts from transgenic Musashi-2 and transgenic Musashi-1 over-expression mice |
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