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Sample GSM1436421 Query DataSets for GSM1436421
Status Public on Jul 16, 2014
Title ST-01012996_S-001146750_8450989135_E
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics treatment: LPS
time collected: 36
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 14, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59404 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #2
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 10.8642415263537 1515.9 392.3425790556 0 35
ILMN_2721178 8.91305444829455 471.1 81.8193834002677 0 35
ILMN_3033922 10.2090210433914 1001.5 238.669344275715 0 33
ILMN_3092673 12.7066818101373 5160.4 710.046952924241 0 37
ILMN_2816356 3.39472967590036 108.4 41.9245752751295 0.17419 28
ILMN_2808939 8.39638645248141 361.4 133.428221182777 0 32
ILMN_2634564 9.40898178953074 620.6 152.825547995091 0 30
ILMN_2737647 3.65864772088354 111.5 39.3975006313852 0.11654 31
ILMN_2734484 8.27411263902269 340.7 86.6280609156179 0 42
ILMN_2952292 7.44830364636572 238.7 66.6389180584439 0 56
ILMN_2699078 2.52623893185876 99 30.444 0.48747 36
ILMN_1213681 7.50508276646166 244 69.9812046195262 0 40
ILMN_2735413 2.45681014598993 98.3 35.6462358181057 0.51253 43
ILMN_2735415 4.3872755538524 120.9 53.3224793028231 0.04637 31
ILMN_2891688 10.4610416302959 1172.3 286.545795332962 0 47
ILMN_2637698 11.1762190302606 1856.1 393.971376767399 0 52
ILMN_2674228 8.75020448788866 432.2 146.465063807039 0 41
ILMN_2601546 3.35116025119768 107.9 37.8634228642895 0.18797 39
ILMN_1230831 4.06836064448767 116.6 46.6021877168873 0.06767 31
ILMN_2848071 4.35872581565114 120.5 46.0360809583092 0.04637 30

Total number of rows: 25697

Table truncated, full table size 1511 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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