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Sample GSM1436433 Query DataSets for GSM1436433
Status Public on Jul 16, 2014
Title ST-01013016_S-001146802_8450989072_A
Sample type RNA
 
Source name Mouse lung tissue
Organism Mus musculus
Characteristics treatment: untreated
time collected: 60
Extracted molecule total RNA
Extraction protocol For mRNA isolation lung tissue was immersed in RNAlater (Qiagen) and total RNA was then extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality (RIN>=7) was confirmed using an Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA from all biological samples was labeled at once using the Ambion TotalPrep-96 RNA Amplification Kit according to the manufacturer's instructions.
 
Hybridization protocol The samples were then blinded, randomized to chip position and hybridized to the Illumina MouseRef-8 v2.0 Expression BeadChips. All chips had one array dedicated as an RNA control.
Scan protocol The chips were read using standard illumina protocols using an Illumina iScan reader.
Data processing VSN normalized, background corrected
 
Submission date Jul 14, 2014
Last update date Jul 16, 2014
Contact name Jen-hwa Chu
E-mail(s) jen-hwa.chu@yale.edu
Organization name Yale University School of Medicine
Department Pulmonary, Critical Care and Sleep Medicine
Street address PO Box 208057
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL6885
Series (2)
GSE59404 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION/ Microarray Experiment #2
GSE59444 CIRCADIAN RHYTHM REPROGRAMMING DURING LUNG INFLAMMATION

Data table header descriptions
ID_REF
VALUE normalized signal intensity
RAW_VALUE raw signal intensity
BEAD_STDERR the standard error of the probe measurements
Detection_Pval the detection p-value of the probe
Avg_NBEADS Number of beads for the probe

Data table
ID_REF VALUE RAW_VALUE BEAD_STDERR Detection_Pval Avg_NBEADS
ILMN_2896528 12.3195516786507 8333.3 1654.75299355553 0 41
ILMN_2721178 10.1331044998908 1949 368.740548841594 0 40
ILMN_3033922 10.5555537935719 2560.5 455.266003628648 0 35
ILMN_3092673 12.7384515242257 11089.8 1515.45428287626 0 32
ILMN_2816356 5.87864760544139 245.7 86.9711370973152 0.00125 28
ILMN_2808939 9.64256958712967 1430.9 358.74271176987 0 28
ILMN_2634564 8.60483203364875 774.7 243.231894906897 0 27
ILMN_2737647 3.21639430973397 157 61.196227824924 0.16416 28
ILMN_2734484 9.68117822340028 1465.6 431.000221846811 0 37
ILMN_2952292 5.76557956677784 238.6 79.34355444521 0.00125 37
ILMN_2699078 1.369453143554 123.9 34.8040950320505 0.81579 31
ILMN_1213681 9.49823521582382 1309.1 320.178371307307 0 37
ILMN_2735413 3.59557042790825 164.9 48.5755823433955 0.10526 38
ILMN_2735415 5.99767197638982 253.8 109.068452047327 0.00125 28
ILMN_2891688 9.82196490539725 1600.3 309.898009958115 0 26
ILMN_2637698 10.8130912458916 3031.3 596.051177922668 0 47
ILMN_2674228 8.57536292724957 762.1 203.484407338744 0 30
ILMN_2601546 3.21144911917991 156.9 57.756 0.16541 36
ILMN_1230831 3.88443743318367 171.3 76.3560869086414 0.06642 42
ILMN_2848071 3.07158342263559 154.1 47.5671607414191 0.19925 29

Total number of rows: 25697

Table truncated, full table size 1513 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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